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Sains Malaysiana 52(12)(2023): 3357-3370
http://doi.org/10.17576/jsm-2023-5212-02
Gene Expression, Growth Performance and Body Composition of Labeo rohita Fingerlings Fed on Polyphenols Supplement
(Pengekspresan Gen, Prestasi Tumbesaran dan Komposisi Badan Anak Labeo rohita Diberi Makan Suplemen Polifenol)
SYED MAKHDOOM HUSSAIN1,*,
MUNIBA JAMIL1, SHAFAQAT ALI2,3, MUHAMMAD MUDASSAR SHAHZAD4,
MAJID HUSSAIN5, NISAR AHMAD6 & DANISH RIAZ4
1Fish Nutrition Lab, Department of Zoology, Government College University, Faisalabad,
Punjab 38000, Pakistan
2Department of Environmental Science, College of
Agriculture and Environmental Sciences, Government College University,
Faisalabad, Punjab 38000, Pakistan
3Department of Biological Sciences and Technology, China Medical University, Taichung 40402, Taiwan 5Department of Fisheries and Aquaculture, University
of Okara, Pakistan
6Department of Zoology, University of Jhang, Pakistan
Diserahkan: 19 Disember 2022/Diterima: 30 November 2023
Abstract
The aim of this
research was to investigate the impacts of polyphenols-supplemented diet on
growth, body composition and gene expressions of insulin-like growth factor
(IGF) and growth
hormone (GH) in Labeo rohita fingerlings fed canola meal-based diets. Two plant extracts; Salvadora persica and Salvadora oleoides were used to assess the efficacy of L. rohita. Twelve test diets,
including control (no supplementation) and supplemented with S. persica, S. oleoidesand
their mixture at the levels of 200, 400 and 800 mg/kg, were formulated. The
gene expression of IGFs and GH was observed to be regulated significantly (p<
0.05) in the liver tissue of fingerlings when fed with supplemented diet. The
highest expression of GH was observed at 200 mg/kg mixture diet. The results of
growth performance showed that maximum weight gain% (215%) and SGR (1.63) was
recorded at 200 mg/kg mixture diet. The results of carcass analysis showed that
they were significantly (p< 0.05) influenced by supplemented diet
when compared with control diet. As growth performance is under genetic
control, the synthesis and release of IGF are stimulated by binding of GH to
receptors present in the liver. Conclusively, the results showed that rohu fingerlings shown the best results of growth performance,
body composition and gene expression of IGF and GH, when fed on mixture of S. persica and S. oleoides at 200 mg/kg.
Keywords: Gene expression; growth hormone;
growth performance; insulin-like growth factors; polyphenols
Abstrak
Matlamat
penyelidikan ini adalah untuk mengkaji kesan diet tambahan polifenol ke atas
tumbesaran, komposisi badan dan pengekspresan gen faktor tumbesaran seperti
insulin (IGF) dan hormon tumbesaran (GH) pada anak Labeo rohita yang
diberi diet berasaskan canola. Dua ekstrak tumbuhan; Salvadora persica dan Salvadora oleoides digunakan untuk menilai keberkesanan L. rohita.
Dua belas ujian diet, termasuk kawalan (tiada suplemen) dan tambahan suplemen S.
persica, S. oleoides serta gabungannya pada tahap 200, 400 dan 800 mg/kg
telah diformulasi. Pengekspresan gen IGF dan GH diperhatikan telah dikawal
dengan ketara (p<0.05) dalam tisu hati anak apabila diberi makanan tambahan.
Pengekspresan tertinggi GH diperhatikan pada diet gabungan 200 mg/kg. Keputusan
prestasi tumbesaran menunjukkan peningkatan% berat badan maksimum (215%) dan
SGR (1.63) direkodkan pada diet gabungan 200 mg/kg. Keputusan analisis karkas
menunjukkan bahawa mereka secara signifikan (p< 0.05) dipengaruhi oleh diet
tambahan jika dibandingkan dengan diet kawalan. Oleh kerana prestasi tumbesaran
berada di bawah kawalan genetik, sintesis dan pembebasan IGF dirangsang oleh
pengikatan GH kepada reseptor yang terdapat dalam hati. Secara konklusif,
keputusan menunjukkan bahawa anak rohu menunjukkan hasil terbaik dalam prestasi
tumbesaran, komposisi badan dan pengekspresan gen IGF dan GH apabila diberi
makan gabungan S. persica dan S. oleoides pada 200 mg/kg.
Kata
kunci: Faktor tumbesaran seperti insulin; hormon tumbesaran; pengekspresan gen;
polifenol; prestasi tumbesaran
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*Pengarang untuk surat-menyurat; email:
drmakhdoomhussain@gcuf.edu.pk
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